心脏缺血预适应的腺苷介导机制研究

丁家望 刘先哲 杨俊 张朝晖 李稳慧 李松 冯义柏 2005-12-13 12:57:51 中华现代内科学杂志 2004年9月第1卷第2期

　　【摘要】 目的 研究心脏缺血预适应对心脏缺血/再灌注（I/R）损伤的保护机制。方法 建立在体家兔心脏I/R模型，测定腺苷含量、CK、LDH漏出率及心肌梗死面积，并观察心肌的组织切片和超微结构，用SAS软件包作成组t检验处理，以观察缺血预适应对I/R心肌损伤的保护效应。结果 缺血预适应组心功能恢复明显好于对照组（P<0.01），CK、LDH漏出率及心肌梗死面积均低于对照组（P<0.01），同时伴有心肌组织腺苷含量增高（P<0.01）。结论 缺血预适应对I/R损伤具有保护作用，腺苷可能介导这种保护效应。

　　关键词 缺血预适应 心肌保护 腺苷

　　Cardioprotection with ischemic preconditioning  mediated by adenosine in rabbits   Ding Jiawang，Liu Xianzhe，Yang Jun，et al.

　　Department of Cardiology，the First Clinical Medical College of Three Gorges University， General Hospital of Yichang，Yichang443003.

　　【Abstract】 Objective The aim of this study was to determine the role of adenosine in the myocardial protection with ischemic preconditioning（IPC）.Methods Protective effect of ischemic preconditioning on myocardium injured by ischemia/reperfusion（I/R）was observed.Results （1）The recovery of heart function in IPC group was better than that of control group（P<0.01）;（2）LDH and CK leakage in IPC group were lower than that of contorl group（P<0.01）;（3）Infarct size in IPC group was smaller than that in control group（P<0.01）;（4）Myocardium adenosine content in IPC group was higher than that in control group（P<0.01）.Conclusion IPC posseses protective effect on myocardium inˉjured by I/R，in which a

denosine plays a crucial role.

　　Key words ischemic preconditioning cardioprotection adenosine

　　本文通过建立在体家兔心脏缺血/再灌注模型，观察了缺血预适应的心肌保护效应，并测定心肌组织腺苷含量，旨在探讨腺苷与缺血预适应心脏保护效应的内在关系。