用激光扫描共聚焦显微镜观察大鼠海马CA3区锥体细胞核酸的变化
健康九九网 www.health99.org 更新时间:2006-9-20 6:01:18
殷 彬 陈耀文 徐虹 林珏龙 2005-12-13 13:07:17 中华中西医杂志 2004年2月第5卷第3期 关键词 海马 DNA RNA 激光扫描共聚焦显微镜 氯氮平 Effects on DNA，RNA meatabolism in hippocampal CA3pyramidalneurons of clozaping rats，studied by laser scanning confocal microscopy Yin Bin，Chen Yaowen，Xu Hong，et al Department of Neurology，the Second Affiliated Hospital of Shantou University，Shantou515041. 【Abstract】 Objective To explore effects on DNA，RNA metabolism in rat hippocampus CA3pyramidal neuˉrons by clozapine.Methods Twenty-four Sprangue-Dawley rats were divided into test and control group，clozapine was given to test group rats intraperitoneally for7days，then LSCM was used to investigate the DNA，RNA change in rats brains slice hippocampus of test and control group.Results The fluorescence pixels of DNA/RNA of average foreground hippocampus CA3pyramidal neurons by clozapine rats was2.684±0.605，The fluorescence pixels of DNA/RNA of average foreground hippocampus CA3pyramidal neurons by clozapine rats was2.954±1.059，There was no significant difference in two groups（P>0.05）.Conclusion It is suggested that clozapine can’t change DNA，RNA in rat hippocampus CA3pyramidal neurons.
【摘要】 目的 探讨中枢神经阻滞剂氯氮平对大鼠海马CA3区锥体细胞DNA和RNA的影响。方法 将24只大鼠分为氯氮平组和生理盐水组，每组12只。利用激光共聚焦显微镜对大鼠的海马神经细胞内DNA和RNA的荧光像素进行观察，并用图像分析技术进行处理，了解海马脑片CA3区锥体细胞DNA、RNA代谢的影响。结果 氯氮平组大鼠海马脑片CA3区锥体细胞内DNA和RNA的荧光像素之比为2.684±0.605，而对照组之比为2.954±1.059，两组间无统计学差异（P>0.05）。结论 氯氮平对大鼠海马CA3区锥体细胞的核酸数量无影响。