正交设计优选微波辅助萃取五味子中有效成分的研究

沈晓琳 阮 俊 黄永林 黄贤清 杨雄辉 2005-12-13 13:13:11 中华中西医杂志 2004年9月第5卷第18期

　　【摘要】 目的 研究微波辅助萃取五味子的提取工艺。方法 以五味子素（五味子甲素和五味子乙素）的提取率为指标，通过正交设计的方案，采用连续微波辐射方法进行微波萃取工艺的优化。结果 微波功率、辐射时间、溶剂浓度、溶剂用量对五味子中有效成分的提取具有交互作用，优化出的最佳工艺条件为:微波功率600W、辐射时间20min、溶剂浓度95%、固液比1∶6。结论 通过验证实验表明，所建立的模型是合理的。

　　关键词 五味子 五味子甲素 五味子乙素 正交设计法 微波辅助萃取

　　Study on optimization of microwave assisted extraction  process for active

　　ingredientsinschisandrachinensis（Turcz）baill with orthogonal design  

　　Shen Xiaolin，Ruan Jun，Huang Yonglin，et al. Institute of Plant，Guangxi541006.

　　【Abstract】 Objective To study the microwave assisted extraction process of Schisandra chinensis（Turcz）Baill.Methods Using the orthogonal design，the optimization process of microave-assisted extraction for total deˉoxyschizandrin and schisandrin B in Schisandra chinensis（Turcz）.Baill was gained by continuous microwave radiation.Results omeparameters，such as microwave power，radiation time，solvent concentration and solvent consumption had interactions to the extraction of active ingredients in Schisandra chinensis（Turcz）Baill.The best optimization proˉcess was obtained as following:600W as the microwave power，20min as radiation time，95%ethanol as solvent and1∶6as the proportion of solid to liquid.Conclusion The established models were proved to be reasonable by the veriˉfied experiment.

　　Key words schisandra chinensis（Turcz）Baill deoxyschizandrin schisandrin Borthogonal design miˉcrowave-assisted extraction

　　1 材料与仪器